Journal: Nutrients

Article Title: Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice

doi: 10.3390/nu13082716

Figure Lengend Snippet: Representative micrographs of immunohistochemical staining of FFARs in formalin-fixed, paraffin-embedded sections of healthy and DSS-treated mouse colon. Figure shows data for FFAR1 ( A , B ), FFAR2 ( C , D ), FFAR3 ( E , F ), and FFAR4 ( G , H ). Arrowheads indicate stained cells. Scale bar = 100 µm. The amount of FFARs ( I ) was presented as an epithelium surface area coverage (%). The pictures were taken in bright field setup with standard color charge-coupled device (CCD) camera (Zeiss AxioCam ICc1; Carl Zeiss, Poznan, Poland). Data are presented as means (bars) with SEM. Significance estimated with the use of unpaired t -test with Welch’s correction n = 2–8; * p < 0.05, as compared to control group.

Article Snippet: The primary rabbit anti-mouse FFAR1 polyclonal antibody (AFR-001; dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR2 polyclonal antibody (AFR-032, dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR3 polyclonal antibody (PA5-97747; dil. 1:1000; Thermo Scientific, Rockford, IL, USA), FFAR4 polyclonal antibody (E-AB-31576; dil. 1:1000; Elabscience, Wuhan, China), and mouse anti-glycerylaldehyde-3-phosphate dehydrogenase (β-actin; sc-47778; dil. 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA) were used.

Techniques: Immunohistochemical staining, Staining, Formalin-fixed Paraffin-Embedded, Control

Journal: Nutrients

Article Title: Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice

doi: 10.3390/nu13082716

Figure Lengend Snippet: Anti-inflammatory effect of FFAR4 agonist GSK137647 at the dose of 1 mg/kg bw, i.p. twice daily is blocked by pretreatment with FFAR4 antagonist AH7614 (5 mg/kg bw, i.p., twice daily). Figure shows data for macroscopic damage score ( A ), colon weight: body weight ratio ( B ), and MPO activity ( C ). Data represent mean ± SEM of n = 8–9 animals per group. Data were analyzed with one-way ANOVA, followed by Newman–Keuls post-hoc test. # p < 0.05, ## p < 0.01, ### p < 0.001 as compared to control group; * p < 0.05, ** p < 0.01 as compared to DSS-treated group; $ p < 0.05 as compared to FFAR agonist.

Article Snippet: The primary rabbit anti-mouse FFAR1 polyclonal antibody (AFR-001; dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR2 polyclonal antibody (AFR-032, dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR3 polyclonal antibody (PA5-97747; dil. 1:1000; Thermo Scientific, Rockford, IL, USA), FFAR4 polyclonal antibody (E-AB-31576; dil. 1:1000; Elabscience, Wuhan, China), and mouse anti-glycerylaldehyde-3-phosphate dehydrogenase (β-actin; sc-47778; dil. 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA) were used.

Techniques: Activity Assay, Control

Journal: Nutrients

Article Title: Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice

doi: 10.3390/nu13082716

Figure Lengend Snippet: Changes in forskolin-stimulated short-circuit current (ΔIsc) in the mouse colon after basolateral application of ( A ) FFAR1, ( B ) FFAR2, ( C ) FFAR3, and ( D ) FFAR4 agonist (10 −5 M). Data represent mean ± SEM, n = 5. Data were analyzed with one-way ANOVA, followed by Newman–Keuls post-hoc test ** p < 0.01, *** p < 0.001 as compared to control; $ p < 0.05, $$$ p < 0.001 as compared to respective FFAR agonist; ## p < 0.01, ### p < 0.001 as compared to DSS.

Article Snippet: The primary rabbit anti-mouse FFAR1 polyclonal antibody (AFR-001; dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR2 polyclonal antibody (AFR-032, dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR3 polyclonal antibody (PA5-97747; dil. 1:1000; Thermo Scientific, Rockford, IL, USA), FFAR4 polyclonal antibody (E-AB-31576; dil. 1:1000; Elabscience, Wuhan, China), and mouse anti-glycerylaldehyde-3-phosphate dehydrogenase (β-actin; sc-47778; dil. 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA) were used.

Techniques: Control

Journal: Nutrients

Article Title: Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice

doi: 10.3390/nu13082716

Figure Lengend Snippet: Changes in veratridine-stimulated short-circuit current (ΔIsc) in the mouse colon after basolateral application of ( A ) FFAR1, ( B ) FFAR2, ( C ) FFAR3, and ( D ) FFAR4 agonist (10 −5 M). Data represent mean ± SEM, n = 5. Data were analyzed with one-way ANOVA, followed by Newman–Keuls post-hoc test. * p < 0.01, ** p < 0.01, *** p < 0.001 as compared to control; $$$ p < 0.001 as compared to respective FFAR agonist; # p < 0.05, ## p < 0.01 as compared to DSS.

Article Snippet: The primary rabbit anti-mouse FFAR1 polyclonal antibody (AFR-001; dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR2 polyclonal antibody (AFR-032, dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR3 polyclonal antibody (PA5-97747; dil. 1:1000; Thermo Scientific, Rockford, IL, USA), FFAR4 polyclonal antibody (E-AB-31576; dil. 1:1000; Elabscience, Wuhan, China), and mouse anti-glycerylaldehyde-3-phosphate dehydrogenase (β-actin; sc-47778; dil. 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA) were used.

Techniques: Control

Journal: Nutrients

Article Title: Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice

doi: 10.3390/nu13082716

Figure Lengend Snippet: Effect of ( A ) FFAR1, ( B ) FFAR2, ( C ) FFAR3, and ( D ) FFAR4 agonist administration (1 mg/kg bw, twice daily; i.p.) on colon permeability in DSS-treated mice. Data represent mean ± SEM, n = 5. Data were analyzed with one-way ANOVA, followed by Newman–Keuls post-hoc test. * p < 0.01, ** p < 0.01, *** p < 0.001 as compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 as compared to DSS.

Article Snippet: The primary rabbit anti-mouse FFAR1 polyclonal antibody (AFR-001; dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR2 polyclonal antibody (AFR-032, dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR3 polyclonal antibody (PA5-97747; dil. 1:1000; Thermo Scientific, Rockford, IL, USA), FFAR4 polyclonal antibody (E-AB-31576; dil. 1:1000; Elabscience, Wuhan, China), and mouse anti-glycerylaldehyde-3-phosphate dehydrogenase (β-actin; sc-47778; dil. 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA) were used.

Techniques: Permeability, Control

Journal: Nutrients

Article Title: Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice

doi: 10.3390/nu13082716

Figure Lengend Snippet: Changes in tight junction-related genes expression in the colon of DSS- and DSS+FFAR4-treated mice. Figure shows data for the expression of occludin ( A ), claudin 1 ( B ), claudin 2 ( C ), claudin 3 ( D ) claudin 4 ( E ), claudin 7 ( F ), claudin 10 ( G ), and claudin 12 ( H ). Values expressed as percent of control group. Data represent mean ± SEM, n ≥ 12. Data were analyzed with one-way ANOVA, followed by Newman–Keuls post-hoc test. * p < 0.05, ** p < 0.01 as compared to control; # p < 0.05, ## p < 0.01 as compared to DSS.

Article Snippet: The primary rabbit anti-mouse FFAR1 polyclonal antibody (AFR-001; dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR2 polyclonal antibody (AFR-032, dil. 1:1000; Alomone Labs, Jerusalem, Israel), FFAR3 polyclonal antibody (PA5-97747; dil. 1:1000; Thermo Scientific, Rockford, IL, USA), FFAR4 polyclonal antibody (E-AB-31576; dil. 1:1000; Elabscience, Wuhan, China), and mouse anti-glycerylaldehyde-3-phosphate dehydrogenase (β-actin; sc-47778; dil. 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA) were used.

Techniques: Expressing, Control

Journal: Journal of Translational Medicine

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation

doi: 10.1186/s12967-022-03526-0

Figure Lengend Snippet: Search for the core gene RGS1 related to cervical cancer immunity and FIGO Stage. A Determine that the soft threshold for network topology analysis is 5. B Generate gene dendrogram and color modules. C Sample clustering heatmap. D Heatmap between module characteristic genes and ESTIMATE results. E Scatter plot of genes in the blue module. F DEGs between FIGO Stage I & II and Stage III & IV. G The core gene-RGS1 was obtained by intersection of DEGs and WGCNA results

Article Snippet: Slides were incubated with RGS1 Polyclonal Antibody (E-AB-11534, Elabscience, China) and HPV16 + 18-E6 Monoclonal Antibody (ab70, abcam, USA) at 4 °C overnight.

Techniques:

Journal: Journal of Translational Medicine

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation

doi: 10.1186/s12967-022-03526-0

Figure Lengend Snippet: Functional enrichment and correlation analysis of key genes. A PPI analysis of genes interacting with RGS1. B GO enrichment analysis of 11 key genes. C Gene–gene correlation analysis. D, E Correlation of RGS1 with CD4 and RGS2. F Correlation between key genes and ESTIMATE analysis. G Correlation between key genes and expression of immune cells (ssGSEA)

Article Snippet: Slides were incubated with RGS1 Polyclonal Antibody (E-AB-11534, Elabscience, China) and HPV16 + 18-E6 Monoclonal Antibody (ab70, abcam, USA) at 4 °C overnight.

Techniques: Functional Assay, Expressing

Journal: Journal of Translational Medicine

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation

doi: 10.1186/s12967-022-03526-0

Figure Lengend Snippet: Clinical relevance of RGS1. A FIGO stage. B T stage. C M stage. D N stage. E DSS event. F PFI event. G OS event

Article Snippet: Slides were incubated with RGS1 Polyclonal Antibody (E-AB-11534, Elabscience, China) and HPV16 + 18-E6 Monoclonal Antibody (ab70, abcam, USA) at 4 °C overnight.

Techniques:

Journal: Journal of Translational Medicine

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation

doi: 10.1186/s12967-022-03526-0

Figure Lengend Snippet: Verification of expression levels of RGS1 in tissues and cells. A The expression of RGS1 was detected by qRT-PCR in tissues. B The expression of RGS1 was detected by immunohistochemistry in tissues. C The expression of RGS1 was detected by immunohistochemistry between HPV-E6-positive cancer tissues and HPV-E6-negative cancer tissues. D The expression of RGS1 was detected by qRT-PCR in cells

Article Snippet: Slides were incubated with RGS1 Polyclonal Antibody (E-AB-11534, Elabscience, China) and HPV16 + 18-E6 Monoclonal Antibody (ab70, abcam, USA) at 4 °C overnight.

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry

Journal: Journal of Translational Medicine

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation

doi: 10.1186/s12967-022-03526-0

Figure Lengend Snippet: Effect of RGS1 knockdown on cervical cancer cells. A qRT-PCR was used to detect the expression of RGS1 after knockdown of RGS1 in HeLa and SiHa cells. B Western Blot was used to detect the expression of RGS1 after knockdown of RGS1 in HeLa and SiHa cells. C CCK8 detected the effect of RGS1 on cell proliferation. D Transwell examined the effects of RGS1 on cell migration and invasion. E FCM was used to detect the effect of RGS1 on apoptosis. F The localization of RGS1 in cells was determined by immunofluorescence

Article Snippet: Slides were incubated with RGS1 Polyclonal Antibody (E-AB-11534, Elabscience, China) and HPV16 + 18-E6 Monoclonal Antibody (ab70, abcam, USA) at 4 °C overnight.

Techniques: Knockdown, Quantitative RT-PCR, Expressing, Western Blot, Migration, Immunofluorescence

Journal: Journal of Translational Medicine

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation

doi: 10.1186/s12967-022-03526-0

Figure Lengend Snippet: The effect of RGS1 on the growth of cervical cancer was detected by nude mice. A The photograph of the tumor. B Tumor growth curve. C Comparison of tumor weight between sh-RGS1 group and sh-NC group. D Comparison of Ki67 expression between sh-RGS1 group and sh-NC group

Article Snippet: Slides were incubated with RGS1 Polyclonal Antibody (E-AB-11534, Elabscience, China) and HPV16 + 18-E6 Monoclonal Antibody (ab70, abcam, USA) at 4 °C overnight.

Techniques: Comparison, Expressing

Journal: Turkish Journal of Pharmaceutical Sciences

Article Title: Evaluation of a Synthetic Polyethyleneimine Based Polymeric Vector for ING4 Gene Delivery to MCF-7 Breast Cancer Cells

doi: 10.4274/tjps.galenos.2023.72430

Figure Lengend Snippet: Protein expression of ING4 with western blot analysis

Article Snippet: Primary antibody incubation was performed using an ING4 polyclonal antibody from Elabscience (E-AB-33309), followed by Horseradish peroxidase (HRP)-conjugated secondary antibody incubation, both conducted in TBS-T containing 0.5% dry milk either at room temperature for 1 hour or at 4 ° C overnight.

Techniques: Expressing, Western Blot

Journal: International Journal of General Medicine

Article Title: Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery

doi: 10.2147/IJGM.S436588

Figure Lengend Snippet: ( A ) Representative immunohistochemical staining images of CST6 and CBX4 expressions; ( B ) statistical chart for HOXA-AS3, CST6 and CBX4. ***P<0.001.

Article Snippet: After that, they were incubated with primary antibody against CST6 (Proteintech, USA, 17076-1-AP) and rabbit anti-human CBX4 polyclonal antibody (Elabscience Biotechnology Co., Ltd., China, E-AB-17696) at 4°C overnight, followed by addition of horseradish peroxidase-labeled secondary antibody polymer in appropriate drops and incubation for 50 min in a greenhouse.

Techniques: Immunohistochemical staining, Staining

Journal: International Journal of General Medicine

Article Title: Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery

doi: 10.2147/IJGM.S436588

Figure Lengend Snippet: Correlations Between HOXA-AS3, CST6, and CBX4 Expressions and Their Correlations with Recurrence of Early Colon Cancer After Surgery

Article Snippet: After that, they were incubated with primary antibody against CST6 (Proteintech, USA, 17076-1-AP) and rabbit anti-human CBX4 polyclonal antibody (Elabscience Biotechnology Co., Ltd., China, E-AB-17696) at 4°C overnight, followed by addition of horseradish peroxidase-labeled secondary antibody polymer in appropriate drops and incubation for 50 min in a greenhouse.

Techniques: Expressing

Journal: International Journal of General Medicine

Article Title: Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery

doi: 10.2147/IJGM.S436588

Figure Lengend Snippet: Effects of HOXA-AS3, CST6, and CBX4 on Recurrence of Early Colon Cancer After Surgery

Article Snippet: After that, they were incubated with primary antibody against CST6 (Proteintech, USA, 17076-1-AP) and rabbit anti-human CBX4 polyclonal antibody (Elabscience Biotechnology Co., Ltd., China, E-AB-17696) at 4°C overnight, followed by addition of horseradish peroxidase-labeled secondary antibody polymer in appropriate drops and incubation for 50 min in a greenhouse.

Techniques: Standard Deviation, Expressing

Journal: International Journal of General Medicine

Article Title: Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery

doi: 10.2147/IJGM.S436588

Figure Lengend Snippet: ROC curves of HOXA-AS3, CST6, and CBX4 expressions in predicting recurrence of early colon cancer after surgery.

Article Snippet: After that, they were incubated with primary antibody against CST6 (Proteintech, USA, 17076-1-AP) and rabbit anti-human CBX4 polyclonal antibody (Elabscience Biotechnology Co., Ltd., China, E-AB-17696) at 4°C overnight, followed by addition of horseradish peroxidase-labeled secondary antibody polymer in appropriate drops and incubation for 50 min in a greenhouse.

Techniques:

Journal: International Journal of General Medicine

Article Title: Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery

doi: 10.2147/IJGM.S436588

Figure Lengend Snippet: Predictive Values of HOXA-AS3, CST6, and CBX4 Expressions for Recurrence of Early Colon Cancer After Surgery

Article Snippet: After that, they were incubated with primary antibody against CST6 (Proteintech, USA, 17076-1-AP) and rabbit anti-human CBX4 polyclonal antibody (Elabscience Biotechnology Co., Ltd., China, E-AB-17696) at 4°C overnight, followed by addition of horseradish peroxidase-labeled secondary antibody polymer in appropriate drops and incubation for 50 min in a greenhouse.

Techniques: Standard Deviation, Expressing